Captain Lionel Messi has pulled out of Barcelona's La Liga clash against Real Betis on Sunday.
Messi has not played a game this season as he also missed Barca's first match of the 2019/2020 season - a 1-0 loss to Athletic Bilbao.
The Argentine superstar missed Barca's pre-season tour of the United States earlier this month with a calf strain, and that setback is yet to go away.
"We need to wait for training, I cannot say with any security if we can count on him." Barca coach Ernesto Valverde said at a pre-match press conference.
"We cannot take a step backwards. If he's not 100 per cent, we must not risk him."
Barca host Betis at Camp Nou on Sunday, their first home game of the new La Liga season.